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Inheritance, heritability, and molecular mapping of QTL for resistance to potato leafhopper (Empoasca fabae Harris) in common beans (Phaseolus vulgaris L.)

Jorge Wilfredo Gonzales C., University of Nebraska - Lincoln


The potato leafhopper (LH) Empoasca fabae Harris is the most important Empoasca species attacking dry beans ( Phaseolus vulgaris L.) in North America. Yield losses of about 20% were reported on susceptible bean varieties in North Platte, NE. The objectives were to determine the inheritance and heritability (h2) of LH injury, and to identify RAPD molecular markers linked to quantitative trait loci (QTL) conferring resistance to LH injury in F2-derived and recombinant inbred line (RIL) populations. ^ Low narrow sense heritability estimates (h2 = 0.29 ± 0.06 and 0.28 ± 0.10, for the F2 populations; and h 2 = 0.25 ± 0.05 and 0.16 ± 0.05, for the RIL populations, respectively) were obtained. In the F2 population from the cross pinto ‘Sierra’ (resistant) x great northern ‘Starlight’, random amplified polymorphic DNA (RAPD) markers AG15.900 on LG 5, and W05.1300 on LG 10 were significantly associated with QTL for resistance using single factor ANOVA and composite interval mapping (CIM) analyses. For the RIL population Belneb-1 x A-55, CIM analysis identified markers AM18.550 and G16.950 on LG 4 with high likelihood ratio (LR) estimates (27.5 and 29.7, respectively) in environment 1. Both markers explained 37.4% of the phenotypic variance. Markers C01.1550 and G17.800 on LG 3 had the highest LR statistics (17.9 and 16.8, respectively) in environment 3. Both markers determined 35.5% of the variance explained. The tight linkage between markers AM18.550 and G16.950 on LG 4 (environment 1) and markers G17.800, and C01.1550 on LG 3 (environment 3), with the QTL for resistance to LH injury, indicates they could be used to develop sequence-characterized amplified regions (SCARs) to facilitate molecular marker assisted selection (MAS). No significant markers were detected in the other RIL population. Use of molecular markers linked to QTL for resistance to LH injury should enhance the efficiency of selection because of the low h2 of the trait. ^

Subject Area

Agriculture, Agronomy|Biology, Molecular

Recommended Citation

Gonzales C., Jorge Wilfredo, "Inheritance, heritability, and molecular mapping of QTL for resistance to potato leafhopper (Empoasca fabae Harris) in common beans (Phaseolus vulgaris L.)" (2001). ETD collection for University of Nebraska - Lincoln. AAI3034376.