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Cloning, mapping, and breeding for disease resistance in common beans (Phaseolus vulgaris L.)

Nedim Mutlu, University of Nebraska - Lincoln

Abstract

Resistance genes containing nucleotide binding site (NBS)-leucine rich repeats (LRR) are the most prevalent types of resistance (R) genes in plants. Kinase-1a domain, in NBS region, is conserved in a few superfamilies, including all NBS-LRR type plant resistance genes. The objectives of this study were: to clone and map members of the kinase-1a gene family, to develop PCR based R-gene specific markers for common bean (Phaseolus vulgaris L), and transfer the quantitative trait loci (QTL) for resistance to common bacterial blight (CBB), caused by Xanthomonas campestris pv. phaseoli , from exotic germplasm XAN-159 to two commercial varieties, pinto ‘Chase’ and great northern (GN) ‘Weihing’. ^ Degenerate primers were used from the most common peptide sequences of kinase-1a and hydrophobic domains (HD) of known NBS-LRR type R genes and EST database. Kinase-1a genes amplified from a cDNA bulk, cloned and mapped via restriction length polymorphism (RFLP). Twenty degenerate primers were used to screen parents BAT 93 and Jalo EEP558 for RGA mapping. Twenty-six kinase-1a gene through RFLP and 32 RGA via PCR were mapped in the common bean recombinant inbred (RI) population BAT93 x Jalo EEP558 where 68 and 72 lines were used, respectively. No kinase-1a gene mapped on linkage group B8 or B10, and no RGA mapped on B4. The linkages to known resistance genes or QTL and important agronomic traits were shown with both kinase-1a and RGA markers. The total of 58 new markers are expected to aid marker assisted selection and future gene cloning studies in common bean. ^ CBB resistance was confirmed, both with greenhouse screening and under natural infection in the field, in advanced BC5 lines for pinto ‘Chase’ and BC3F2 plants for GN ‘Weihing’. The linkage to V locus that is associated with small seed size and black seed coat color was broken in some BC4 and BC5 pinto ‘Chase’ lines where high resistance to CBB was combined with acceptable seed size and pinto seed coat color. ^

Subject Area

Agriculture, Agronomy|Biology, Genetics

Recommended Citation

Mutlu, Nedim, "Cloning, mapping, and breeding for disease resistance in common beans (Phaseolus vulgaris L.)" (2002). ETD collection for University of Nebraska - Lincoln. AAI3045527.
http://digitalcommons.unl.edu/dissertations/AAI3045527

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