Off-campus UNL users: To download campus access dissertations, please use the following link to log into our proxy server with your NU ID and password. When you are done browsing please remember to return to this page and log out.
Non-UNL users: Please talk to your librarian about requesting this dissertation through interlibrary loan.
Construction of genetic linkage maps and locations of disease resistant and plant architectural loci in common beans using random amplified polymorphic DNA (RAPD) markers
Abstract
Common bacterial blight (CBB), web blight (WB), and rust, incited by the bacterial pathogen Xanthomonas campestris pv. phaseoli (Smith) Dye (Xcp), fungal pathogens Thanatephorus cucumeris (Frank) Donk (Tc) and Uromyces appendiculatus (Pers.:Pers) Unger (Ua), respectively, are important diseases in Phaseolus vulgaris L. The objectives of the research were: to construct linkage maps using RAPD markers in P. vulgaris recombinant inbred (RI) lines derived from the cross 'PC-50' x XAN-159 to locate CBB resistance loci (QTLs) of different plant parts to different Xcp strains and an abaxial leaf pubescence locus and from the cross BAC-6 x HT-7719 to locate CBB and WB resistance loci (QTLs), a specific rust resistance locus and plant architectural traits (QTLs). Ten linkage groups using 180 RAPDs and the abaxial leaf pubescence locus were identified in 70 RI lines from the cross 'PC-50' x XAN-159. Eight linkage groups using 89 RAPDs and the rust resistance locus were identified in 128 RI lines from the cross BAC-6 x HT-7719. Regression analysis and the interval mapping method using MAPMAKER/QTL were used to identify genomic regions involved in the genetic control of the traits. A chromosome region on linkage group six carried factors influencing reactions to two Xcp strains in different plant parts in the cross 'PC-50' x XAN-159. In the cross BAC-6 x HT-7719 a chromosome region on linkage group 9 carried factors influencing WB and CBB resistance in pods, first trifoliolate, and later developed leaves to Xcp EK-11. This study illustrates the usefulness of RI lines populations and RAPD markers for mapping loci for complex traits such as CBB and WB resistance and plant architectural traits. It suggests that even though a complex of genes is associated with resistance to Xcp of different plant parts in common beans, one major gene was found to control a significant proportion of the phenotypic variation for resistance to different strains in different plant organs.
Subject Area
Plant pathology|Genetics|Molecular biology
Recommended Citation
Jung, Geunhwa, "Construction of genetic linkage maps and locations of disease resistant and plant architectural loci in common beans using random amplified polymorphic DNA (RAPD) markers" (1995). ETD collection for University of Nebraska-Lincoln. AAI9538637.
https://digitalcommons.unl.edu/dissertations/AAI9538637