Distinct and Shared Determinants of Cardiomyocyte Contractility in Multi-Lineage Competent Ethnically Diverse Human iPSCs

Authors

Martin L. Tomov, State University of New York Polytechnic Institute
Zachary T. Olmsted, State University of New York Polytechnic Institute
Haluk Dogan, University of Nebraska-LincolnFollow
Eda Gongorurler, Istanbul University Faculty of Medicine
Maria Tsompana, State University of New York Buffalo
Hasan H. Otu, University of Nebraska-LincolnFollow
Michael Buck, State University of New York Buffalo
Eun-Ah Chang, Eulji University
Jose Cibelli, Michigan State University
Janet L. Paluh, State University of New York Polytechnic InstituteFollow

Date of this Version

2016

Citation

Sci. Rep. 6, 37637

Comments

© The Author(s) 2016

Open access

DOI: 10.1038/srep37637

Abstract

The realization of personalized medicine through human induced pluripotent stem cell (iPSC) technology can be advanced by transcriptomics, epigenomics, and bioinformatics that inform on genetic pathways directing tissue development and function. When possible, population diversity should be included in new studies as resources become available. Previously we derived replicate iPSC lines of African American, Hispanic-Latino and Asian self-designated ethnically diverse (ED) origins with normal karyotype, verified teratoma formation, pluripotency biomarkers, and tri-lineage in vitro commitment. Here we perform bioinformatics of RNA-Seq and ChIP-seq pluripotency data sets for two replicate Asian and Hispanic-Latino ED-iPSC lines that reveal differences in generation of contractile cardiomyocytes but similar and robust differentiation to multiple neural, pancreatic, and smooth muscle cell types. We identify shared and distinct genes and contributing pathways in the replicate ED-iPSC lines to enhance our ability to understand how reprogramming to iPSC impacts genes and pathways contributing to cardiomyocyte contractility potential.