Development of methods to evaluate susceptibility of soybean aphid to imidacloprid and thiamethoxam at lethal and sublethal concentrations

Authors

Leonardo C. Magalhaes, University of Nebraska-Lincoln
Thomas E. Hunt, University of Nebraska, Haskell Agricultural Laboratory, Concord, NEFollow
Blair D. Siegfried, University of Nebraska-LincolnFollow

Date of this Version

5-2008

Comments

Published in Entomologia Experimentalis et Applicata 128:2 (2008), pp. 330–336; doi 10.1111/j.1570-7458.2008.00718.x Copyright © 2008 Leonardo C. Magalhaes, Thomas E. Hunt, and Blair D. Siegfried. Journal compilation © 2008 The Netherlands Entomological Society; published by John Wiley & Sons. Used by permission. http://www.interscience.wiley.com/jpages/0013-8703

Abstract

The soybean aphid, Aphis glycines Matsumura (Homoptera: Aphididae), is a recent introduction (2000) from Asia and has become a serious soybean [Glycine max (L.) Merr. (Fabaceae)] pest in North America. Seed treatments using the neonicotinoid insecticides, imidacloprid and thiamethoxam, have been suggested as a method of control, and the use of these insecticides is becoming widespread. As a consequence, there is increased potential to select for resistance to these compounds. In the case of soybean aphids, baseline susceptibility to neonicotinoid insecticides and standardized methods for bioassay are lacking. A bioassay technique that uses excised soybean leaves immersed in an insecticide solution was developed to determine systemic insecticidal activity at lethal and sublethal concentrations. Mortality and population growth inhibition were evaluated after 7 days. Life table parameters were calculated by exposing 1-day-old aphids to three concentrations of thiamethoxam. Aphid mortality and nymph production were recorded daily until the entire cohort collapsed. Soybean aphid age-specific survivorship, fecundity, net reproductive rate, longevity, intrinsic rate of increase, discrete daily growth rate, and life expectancy were all significantly reduced at higher thiamethoxam concentrations. Soybean aphid response to both insecticides was similar, and both compounds were very toxic with LC₅₀s of 31.3 and 16.9 ng ml⁻¹ and EC₅₀s of 6.3 and 5.4 ng ml⁻¹ for imidacloprid and thiamethoxam, respectively. These results indicate that the methods developed in this study had negligible impact on the life table estimates measured and can be used to develop a baseline of susceptibility as a benchmark for subsequent resistance monitoring. Given the rapid and widespread adoption of this new insecticide class, vigilant monitoring for changes in susceptibility will be essential to its long-term sustainability.