Evaluation of Greenbug and Yellow Sugarcane Aphid Feeding Behavior on Resistant and Susceptible Switchgrass Cultivars

Authors

Kyle G. Koch, University of Nebraska-LincolnFollow
Teresa Donze-Reiner, West Chester University of PennsylvaniaFollow
Lisa M. Baird, University of San DiegoFollow
Joe Louis, University of Nebraska–LincolnFollow
Keenan L. Amundsen, University of Nebraska-LincolnFollow
Gautam Sarath, USDAFollow
Jeff Bradshaw, University of Nebraska-LincolnFollow
Tiffany Heng-Moss, University of Nebraska - LincolnFollow

Document Type

Article

Date of this Version

2018

Citation

BioEnergy Research (2018) 11:480–490

Comments

Copyright Springer Science+Business Media

This document is a U.S. government work and is not subject to copyright in the United States.

https://doi.org/10.1007/s12155-018-9914-3

Abstract

Switchgrass (Panicum virgatum L.) is an emerging biofuel crop that serves as host for aphids. To discern the effects of plant age and possible resistance mechanisms, the feeding behavior of greenbugs (Schizaphis graminum Rondani.) and the yellow sugarcane aphid (Sipha flava Forbes.) was monitored on three diverse switchgrasses by the electrical penetration graph (EPG) technique. Callose deposition and genes associated with callose metabolism were also analyzed to discern their association with plant resistance. There was a strong host effect on greenbugs feeding on lowland cultivar Kanlow at the V3 stage of development, as compared to the greenbug-susceptible upland cultivar Summer and plants derived from Kanlow (♂) × Summer (♀) (K×S) crosses. These data confirmed that Kanlow at the V3 stage had antibiosis to greenbugs, which was absent in the Summer and K×S plants. In contrast, similar effects were not observed for yellow sugarcane aphids, excluding significant differences in the time to first probe on Kanlow plants at the V1 stage and reduction in time spent on pathway processes on Kanlow plants at the V3 stage. These data demonstrated that Kanlow plants may have multiple sources of resistance to the two aphids, and possibly some were phloem based. Microscopy of leaf sections stained with aniline blue for callose was suggestive of increased callose deposition in the sieve elements in Kanlow plants relative to Summer and K×S plants. RT-qPCR analysis of several genes associated with callose metabolism in infested plants was equivocal. Overall, these studies suggest the presence of multiple defense mechanisms against aphids in Kanlow plants, relative to Summer and K×S plants.