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Nitrogen gas (N2) comprises nearly 80 percent of total atmospheric gases, yet most organisms are unable to use N2 as a source of nitrogen. Legumes, such as soybean, are able to capture atmospheric nitrogen and utilize it through the process of nitrogen fixation. This NebGuide is part one of a two-part series on soybean inoculation. Here, we will investigate how soybean inoculation occurs and which environmental conditions impact nitrogen fixation.