Determination of 4,4′-Dinitrocarbanilide (DNC), the Active Component of the Antifertility Agent Nicarbazin, in Chicken, Duck, and Goose Plasma

Authors

Thomas M. Primus, U.S. Department of Agriculture/Animal and Plant Health Inspection Service/National Wildlife Research Center
Dennis J. Kohler, USDA/APHIS/WS National Wildlife Research CenterFollow
Margaret A. Goodall, U.S. Department of Agriculture/Animal and Plant Health Inspection Service/National Wildlife Research Center
Christi Yoder, U.S. Department of Agriculture/Animal and Plant Health Inspection Service/National Wildlife Research Center
Doreen Griffin, U.S. Department of Agriculture/Animal and Plant Health Inspection Service/National Wildlife Research Center
Lowell A. Miller, U.S. Department of Agriculture/Animal and Plant Health Inspection Service/National Wildlife Research CenterFollow
John J. Johnston, U.S. Department of Agriculture/Animal and Plant Health Inspection Service/National Wildlife Research CenterFollow

Date of this Version

July 2001

Comments

Published in J. Agric. Food Chem. 2001, 49, 3589-3593.

Abstract

4,4′-Dinitrocarbanilide (DNC) was extracted from chicken, duck, and goose plasma and isolated by reversed-phase high-performance liquid chromatography. DNC was detected by ultraviolet absorbance at 347 nm and quantified by comparison to a calibration standard. Recovery data were determined by analyzing DNC-fortified control plasma. The mean recovery of DNC in fortified chicken plasma samples was 99.7 ± 1.9% for 0.18 and 9.1 ppm DNC, and in fortified duck and goose plasma samples was 99.5 ± 4.9% and 101.4 ± 4.5%, respectively, for 0.18, 9.1, and 18 ppm DNC.