Determination of 4,4'-Dintrocarbanilide (DNC), a Component of Nicarbazin, in Canada Goose (Branta canadensis) Eggshells Using High-Performance Liquid Chromatography

Authors

Randal S. Stahl, USDA-APHIS-Wildlife ServicesFollow
Kurt C. VerCauteren, USDA-APHIS-Wildlife ServicesFollow
Teresa L. Buettgenbach, USDA/APHIS/WS National Wildlife Research Center
John J. Johnston, USDA/APHIS/WS National Wildlife Research CenterFollow

Document Type

Article

Date of this Version

June 2003

Comments

Published in J. Agric. Food Chem. 2003, 51, 1130-1135. Permission to use.

Abstract

A method was developed using high-performance liquid chromatography to assay 4,4'-dinitrocarbanilide (DNC), the active ingredient in Nicarbazin, in eggshells collected from Canada geese fed a formulated feed fortified with Nicarbazin at doses of 0, 125, 250, and 500 μg/g. The method was developed using chicken eggshells fortified with DNC. The method was used to quantify DNC in both the shell-associated membranes and the calcified shell extracellular matrix. These values were compared to those obtained for a composite sample consisting of both the membranes and the calcified shell extracellular matrix. The validated method was used to quantify DNC in eggshells from geese fed fortified feed to ascertain the effect of Nicarbazin feed concentration on shell DNC concentration. DNC levels in the eggshells were highly correlated with feed dose.