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The study investigated the relationship between the size and quality of information sources and the use of library services in the university libraries in the southeast of Nigeria. Two objectives, two research questions and two hypotheses guided this study. The population for this study was made up of 12,500 registered users out of which a sample of 2000 was taken using proportionate sampling technique. Data were collected using the questionnaire and were analyzed through the use of frequency tabulation and percentages. The hypotheses were tested using statistical method of Pearson Product Moment Correlation (PPMC) analysis and Multiple Regression. Analysis was done with the use of computer programme of Statistical Package for Social Science (SPSS)version10.0 The hypotheses testing were conducted at 0.05 level of significance (p<0.05) for rejection or retention. The size and quality of information sources therefore have significant relationship with the use of library services in the university libraries in the southeast Nigeria. Recommended adequate funding to acquire adequate size and good quality information sources as well as the provision of ICT and Internet facilities to encourage the use of library services.