Mechanical & Materials Engineering, Department of

 

Document Type

Article

Date of this Version

7-7-2022

Citation

Methods Mol Biol. 2020 ; 2050: 91–97. doi:10.1007/978-1-4939-9740-4_10.

Comments

PMCID: PMC9262042

Abstract

Electroporation is a common method of transfection due to its relatively low risk and high transfection efficiency. The most common method of electroporation is bulk electroporation which is easily performed on large quantities of cells yet results in variable levels of viability and transfection efficiency across the population. Localized electroporation is an alternative that can be administered on a similar scale but results in much more consistent with higher quality transfection and higher cell viability. This paper discusses the creation and use of a simple and cost-effective device using porous membrane for performing localized electroporation.

Share

COinS