Natural Resources, School of
Date of this Version
2-1-2003
Citation
Memorias do Instituto Oswaldo Cruz 98: 67–69. doi: 10.1590/s0074-02762003000900013
Abstract
A molecular paleoparasitological diagnostic approach was developed for Enterobius vermicularis. Ancient DNA was extracted from 27 coprolites from archaeological sites in Chile and USA. Enzymatic amplification of human mtDNA sequences confirmed the human origin. We designed primers specific to the E. vermicularis 5S ribosomal RNA spacer region and they allowed reproducible polymerase chain reaction identification of ancient material. We suggested that the paleoparasitological microscopic identification could accompany molecular diagnosis, which also opens the possibility of sequence analysis to understand parasite-host evolution.
Included in
Biological and Physical Anthropology Commons, Natural Resources and Conservation Commons, Parasitic Diseases Commons, Parasitology Commons
Comments
Copyright 2003, the authors. Used by permission.
Citation: Iñiguez AM, Reinhard KJ, Araújo A, Ferreira LF, Vicente ACP (2003) Enterobius vermicularis ancient DNA from North and South American human coprolites. Memories of the Instituto do Oswaldo Cruz 98:67-69.Citation: Iñiguez AM, Reinhard KJ, Araújo A, Ferreira LF, Vicente ACP (2003) Enterobius vermicularis ancient DNA from North and South American human coprolites. Memorias do Instituto do Oswaldo Cruz 98:67-69. Detected language : Portuguese