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Blood and tissue samples from 66 white-tailed deer (Odocoileus virginanus) and 69 mule deer (Odocoileus hemionus) were examined by isoelectric focusing on polyacrylamide gel slabs in the pH range 6-9. Blood was differentiated via a general protein stain. Tissue differentiation required use of esterase patterns rather than general protein patterns. Esterase patterns of white-tailed and mule deer tissue differed considerably from each other in both number and location of esterase bands. Protein patterns for blood were not as distinctive as the esterase patterns were for tissue, but white-tailed and mule deer could still be differentiated from each other. Some variation in individual patterns of blood and tissue was observed in both white-tailed and mule deer.