First Report of Goss's Bacterial Leaf Blight and Wilt of Corn Caused by Clavibacter michiganensis subsp. nebraskensis in North Dakota

Authors

• A. Friskop, North Dakota State UniversityFollow
• K. Kinzer, North Dakota State University
• M. McConnell, North Dakota State University
• Z. Liu, North Dakota State University
• K. A. Korus, University of Nebraska-LincolnFollow
• A. Timmerman, University of Nebraska-LincolnFollow
• T. Jackson, University of Nebraska-Lincoln

Document Type

Article

Date of this Version

12-2014

Citation

Plant Disease, December 2014, Volume 98, Number 12, Page 1739 doi: 10.1094/PDIS-04-14-0397-PDN

Published by The American Phytopathological Society

Comments

Open Access.

Abstract

In August of 2011, the North Dakota State University Plant Diagnostic Lab received a hybrid corn (Zea mays) leaf sample from Burleigh County in southcentral North Dakota (ND). The leaf had long, irregular, water-soaked lesions consistent with Goss's leaf blight of corn. Using a light microscope at 10× magnification, bacterial streaming was observed from the excised edge of leaf tissue. A bacterial suspension was created, streaked onto a semi-selective CNS medium (1), and incubated at 22°C. Dark yellow-orange colonies appeared on the medium after 5 days. Single colonies were subcultured onto additional CNS media. To verify the identity of the bacterial isolate, PCR amplification of the 16S ribosomal DNA from this isolate along with a known Clavibacter michiganensis spp. nebraskensis (Cmn) isolate collected in Indiana (4) was performed using the eubacterial universal primers 27f and 1525r (3). The 1,431-bp 16S rDNA region was obtained for each isolate and they were compared with each other and with those deposited in NCBI GenBank.