Variants of Triticum mosaic virus Isolated From Wheat in Colorado Show Divergent Biological Behavior

Authors

Dallas L. Seifers, Kansas State UniversityFollow
Satyanarayana Tatineni, University of Nebraska-LincolnFollow
Roy French, University of Nebraska-LincolnFollow

ORCID IDs

Satyanarayana Tatineni

Document Type

Article

Date of this Version

2013

Citation

Seifers, D. L., Tatineni, S., and French, R. 2013. Variants of Triticum mosaic virus isolated from wheat in Colorado show divergent biological behavior. Plant Dis. 97:903-911.

Comments

U. S. government work.

Abstract

Triticum mosaic virus (TriMV) is a recently discovered virus infecting wheat. We compared Colorado isolates C10-492 and C11-775 with the 06-123 isolate. Comparisons were made using enzyme-linked immunosorbent assay (ELISA), infectivity assay, host range, dry weight (DW), inoculation of ‘Mace’ wheat with temperature-sensitive resistance to Wheat streak mosaic virus, and the deduced amino acid sequence of the coat proteins (CP) and P1 proteins. Both C10-492 and C11-775 infected ‘Gallatin’ barley and, when compared with 06-123, had significantly reduced ELISA values and virus titers in wheat. Both Colorado isolates caused symptomless infections in Mace, whereas 06- 123 caused mosaic symptoms. The amino acid sequences of the CP differed by two and one amino acids for C10-492 and C11-775, respectively, compared with 06-123. The sequence of C10-492 differed from C11-775 by one amino acid. The P1 amino acid sequence of C10-492 and C11-775 differed from 06-123 by three and one amino acids, respectively. The C10-492 and C11-775 isolates reduced DW significantly in ‘Karl 92’ but significantly less than 06-123. In ‘2317’ wheat, the Colorado isolates did not consistently cause significant reduction in DW, while 06-123 did. The data collectively indicate that C10-492 and C11-775 are isolates of TriMV showing biological behavior diverse from that of 06-123.