Expression of the tobacco β- 1,3-glucanase gene, PR-2d, following induction of SAR with Peronospora tabacina

Authors

Deanna L. Funnell, University of Nebraska-LincolnFollow
Christopher B. Lawrence, Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA
Jeffrey F. Pedersen, University of Nebraska-LincolnFollow
Christopher L. Schardl, University of Kentucky, Lexington, KY

Document Type

Article

Date of this Version

December 2004

Comments

Published in Physiological and Molecular Plant Pathology 65:6 (December 2004), pp. 285-296.

Abstract

Systemic acquired resistance (SAR) is induced following inoculation of Peronospora tabacina sporangia into the stems of Nicotiana tabacum plants highly susceptible to the pathogen. Previous results have shown that accumulation of acidic P-1,3-glucanases (PR-2's) following induction of SAR by P. tabacina may contribute to resistance to P. tabacina. We showed that up-regulation of the PR-2 gene, PR-2d, following stem inoculation with P. tabacina, is associated with SAR. Studies using plants transformed with GUS constructs containing the full length promoter from PR-2d or promoter deletions, provided evidence that a previously characterized regulatory element that is involved in response to salicylic acid (SA), may be involved in regulation of PR-2d following induction of SAR with P. tabacina. This work provides evidence that regulation of PR-2 genes during P. tabacina-induced SAR may be similar to regulation of these genes during infection of N-gene tobacco by TMV or following exogenous application of SA, and provides further support for the role of SA in regulation of genes during P. tabacina-induced SAR.