A Preliminary Linkage Map of the Hard Tick, Ixodes Scapularis

Authors

A. J. Ullmann, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA
J. Piesman, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA
M. C. Dolan, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA
W. C. Black IV, Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1677, USA

Date of this Version

2003

Comments

Published in Insect Molecular Biology (2003) 12 (2), 201–210.

Abstract

Blackwell Publishing Ltd. A linkage map of the Ixodes scapularis genome was constructed, based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence-Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F₁ intercross progeny from a single, field-collected P₁ female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of ~ 10 ⁹ bp, the relationship of physical to genetic distance was found to be ~ 300 kb/cM in the I. scapularis genome.