A Preliminary Linkage Map of the Hard Tick, Ixodes Scapularis

Authors

• A. J. Ullmann, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA
• J. Piesman, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA
• M. C. Dolan, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA
• W. C. Black IV, Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1677, USA

Date of this Version

2003

Comments

Published in Insect Molecular Biology (2003) 12 (2), 201–210.

Abstract

Blackwell Publishing Ltd. A linkage map of the Ixodes scapularis genome was constructed, based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence-Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F₁ intercross progeny from a single, field-collected P₁ female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of ~ 10 ⁹ bp, the relationship of physical to genetic distance was found to be ~ 300 kb/cM in the I. scapularis genome.