ATA2-Mediated Amino Acid Uptake Following Partial Hepatectomy is Regulated by Redistribution to the Plasma Membrane

Authors

Thomas L. Freeman, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE, USA
Geoffrey M. Thiele, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE, USAFollow
Dean J. Tuma, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE, USA
Tina K. Machu, Department of Pharmacology, Texas Tech University Health Sciences Center, Lubbock, TX, USA
Mark E. Mailliard, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE, USA

Date of this Version

2002

Comments

Published in Archives of Biochemistry and Biophysics 400 (2002) 215–222.

Abstract

System A, the Na⁺-dependent amino acid transport activity, is encoded by the ATA2 gene and up-regulated following partial hepatectomy (PH), and its competitive inhibition interferes with liver regeneration. Rabbit polyclonal antibody was raised against a portion of the ATA2 gene product followed by immunodetection of ATA2 in isolated liver plasma membrane and lysate. The level of ATA2 increased in the plasma membrane following PH, while the relatively high quantity of ATA2 found in liver lysate remained constant. We also have shown that Northern analysis of steady-state ATA2 mRNA revealed no significant change following PH. These data show that ATA2-mediated transport is not regulated by the steady-state level of ATA2 mRNA but is regulated by the amount of ATA2 and redistribution to the plasma membrane. We hypothesize that ATA2 activity is regulated by recruitment of ATA2 protein from an intracellular compartment. In addition, the pattern of expression of System A activity in oocytes, transport kinetics, and sensitivity to chemical modification indicate the presence of a second System A isoform in liver that differs substantially from ATA2.