Immunogenicity of combination DNA vaccines for Rift Valley fever virus, tick-borne encephalitis virus, Hantaan virus, and Crimean Congo hemorrhagic fever virus

Authors

Connie Schmaljohn, Division U.S. Army Medical Research Institute of Infectious DiseasesFollow
Kristin Spik, United States Army Medical Research Institute of Infectious Diseases
Amy Shurtleff, United States Army Medical Research Institute of Infectious Diseases
Anita McElroy, United States Army Medical Research Institute of Infectious Diseases
Mary Guttieri, United States Army Medical Research Institute of Infectious DiseasesFollow
Jay Hooper, United States Army Medical Research Institute of Infectious Diseases

Date of this Version

2006

Citation

Vaccine 24 (2006) 4657–4666

Comments

This article is a U.S. government work, and is not subject to copyright in the United States.

Abstract

DNAvaccines for RiftValley fever virus (RVFV), Crimean Congo hemorrhagic fever virus (CCHFV), tick-borne encephalitis virus (TBEV), and Hantaan virus (HTNV), were tested in mice alone or in various combinations. The bunyavirus vaccines (RVFV, CCHFV, and HTNV) expressed Gn and Gc genes, and the flavivirus vaccine (TBEV) expressed the preM and E genes. All vaccines were delivered by gene gun. The TBEV DNA vaccine and the RVFV DNA vaccine elicited similar levels of antibodies and protected mice from challenge when delivered alone or in combination with other DNAs. Although in general, the HTNV and CCHFV DNA vaccines were not very immunogenic in mice, there were no major differences in performance when given alone or in combination with the other vaccines