In Vitro Expression of Full-Length and Truncated Bovine Respiratory Syncytial Virus G Proteins and Their Antibody Responses in BALB/c Mice

Authors

Ryan P. Brady, University of Nebraska-Lincoln
Christina L. Topliff, University of Nebraska-LincolnFollow
Clayton L. Kelling, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

September 2004

Comments

Published in Vaccine 22:27-28 (September 9, 2004), pp. 3762–3768; 10.1016/j.vaccine.2004.03.020 Copyright © 2004 Elsevier Ltd. Used by permission. http://www.sciencedirect.com/science/journal/0264410X A contribution of the University of Nebraska Agricultural Research Division, Lincoln, NE 68583, USA. Journal Series No. 14293.

Abstract

Bovine respiratory syncytial virus (BRSV) is a primary cause of lower respiratory tract disease in calves. Protection is incomplete following vaccination or natural infection, as re-infections are common. The objectives of this study were to create plasmid DNA constructs encoding the full-length, secreted, or conserved region of the BRSV G glycoprotein, and to compare and evaluate their expression in cell culture and potential to induce antibody responses in BALB/c mice. Transfection of COS-7 cells with plasmid DNA resulted in expression of the BRSV G region from each of the plasmid DNA constructs. Following inoculation of BALB/c mice with plasmid DNA, a significant and equivalent anti-BRSV G IgG response was elicited to the full-length and truncated BRSV G proteins. These constructs may be used to study host pathological and immunological responses.