David R. Holding
Date of this Version
Ren, Ying,"Generation and Evaluation of Modified opaque-2 (o2) Popcorn Suggests a Route to Quality Protein Popcorn (QPP)" (2018). Theses, Dissertations, and Student Research in Agronomy and Horticulture.
I have been working on a Quality Protein Popcorn breeding project where QPM conversion is carried out simultaneously for several elite popcorn germplasms. During my study in the graduate program, I led the following aspects of the Quality Protein Popcorn Breeding Project:
1. Identified suitable QPMs as opaque-2 allele donors.
2.Examined the feasibility of quick introgression of the opaque-2 allele into popcorn lines via marker-assisted selection.
3. Monitored modification by SDS-PAGE zein profiling and light box phenotypic selection to make sure multiple modifier loci for opaque-2 were incorporated each time generation advancement was carried out.
4. Carried out high throughput DNA extraction using the BioSprint Workstation and performed genotyping to guide field selection.
5. Principle Component Analysis (PCA) of amino acid profiles from UPLC (Ultra Performance Liquid Chromatography) of candidate QPP lines along with corresponding parental lines (QPM and popcorn). (in collaboration with Dr. Ruthie Angelovici from University of Missouri Columbia, MO)
Advisor: David R. Holding