Agronomy and Horticulture Department


Date of this Version



A THESIS Presented to the Faculty of The Graduate College at the University of Nebraska In Partial Fulfillment of Requirements For the Degree of Master of Science, Major: Horticulture, Under the Supervision of Professor Paul E. Read. Lincoln, Nebraska: December, 2010
Copyright 2010 Brant B. Bigger


Propagation of the Vitis aestivalis cultivar 'Norton‘ (syn='Cynthiana‘) through traditional woody cuttings has been difficult. Rooting of woody cuttings has been a major hindrance in propagating this cultivar and providing enough plants to meet grower needs. In vitro propagation offers another method of increasing plant material. Cultures were established and maintained on Murashige and Skoog (MS) medium supplemented with 4 M 6-benzyladenine (BA) and thiamine at 0.5 mg•L-1 and solidified with Difco-Bacto agar at 7.5 g•L-1. The objectives of this study were to determine optimal methods for in vitro production and ex vitro establishment of 'Norton‘ plantlets. A factorial treatment with 4 concentrations of BA (0, 2, 4 & 8 µM) and 2 concentrations of naphthaleneacetic acid (NAA) (0 & 0.5 µM) was used for the multiplication study. Plantlets were acclimatized to ex vitro conditions without in vitro rooting. Plantlets were rooted ex vitro either with or without a 1000 ppm (0.1%) indolebutric acid (IBA) basal dip for 5 seconds. Auxin did not have a significant effect on explant growth or plantlet rooting. This demonstrates an improved method of in vitro propagation and acclimatization for efficient multiplication of 'Norton‘ grapevines.
Advisor: Paul E. Read