Reversing resistance to tembotrione in an Amaranthus tuberculatus (var. rudis) population from Nebraska, USA with cytochrome P450 inhibitors

Authors

• Maxwel C. Oliveira, University of Nebraska-LincolnFollow
• Todd A. Gaines, Colorado State University in Fort CollinsFollow
• Franck E. Dayan, Colorado State University in Fort CollinsFollow
• Eric L. Patterson, Colorado State University in Fort CollinsFollow
• Amit J. Jhala, University of Nebraska-LincolnFollow
• Stevan Z. Knezevic, University of Nebraska-LincolnFollow

ORCID IDs

Amit J. Jhala

Document Type

Article

Date of this Version

2017

Citation

Published in Pest Management Science (2017)

doi 10.1002/ps.4697

Comments

Copyright © 2017 Society of Chemical Industry; published by John Wiley Inc. Used by permission.

Abstract

Background: A population of Amaranthus tuberculatus (var. rudis) was confirmed resistant to 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibitor herbicides (mesotrione, tembotrione, and topramezone) in a seed corn/soybean rotation in Nebraska. Further investigation confirmed a non-target-site resistance mechanism in this population. The main objective of this study was to explore the role of cytochrome P450 inhibitors in restoring the efficacy of HPPD-inhibitor herbicides on the HPPD-inhibitor resistant A. tuberculatus population from Nebraska, USA (HPPD-R).

Background: A population of Amaranthus tuberculatus (var. rudis) was confirmed resistant to 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibitor herbicides (mesotrione, tembotrione, and topramezone) in a seed corn/soybean rotation in Nebraska. Further investigation confirmed a non-target-site resistance mechanism in this population. The main objective of this study was to explore the role of cytochrome P450 inhibitors in restoring the efficacy of HPPD-inhibitor herbicides on the HPPD-inhibitor resistant A. tuberculatus population from Nebraska, USA (HPPD-R).

Results: Enhanced metabolism via cytochrome P450 enzymes is the mechanism of resistance in HPPD-R. Amitrole partially restored the activity of mesotrione, whereas malathion, amitrole, and piperonyl butoxide restored the activity of tembotrione and topramezone in HPPD-R. Although corn was injured through malathion followed by mesotrione application a week after treatment, the injury was transient, and the crop recovered.

Includes supplementary file.