Agronomy and Horticulture Department


Date of this Version



Published in SCIENCE September 16, 1949, Vol. 110.


The application of one- and two-dimensional paper chromatography to the separation of small amounts of many kinds of compounds has become a well-established and useful technique (1, 2, 3, 5, 6). In general these methods do not handle sufficiently large quantities of materials for isolation and chemical identification. Large columns of starch and other materials have been used satisfactorily for handling larger quantities of mixtures (4). Starch columns proved to be unsatisfactory, how- ever, for isolation of certain substances under investigation in this laboratory. As a consequence, a new and simple apparatus and technique have been developed, using a pile of filter paper disks as the absorbing column.