Agronomy and Horticulture Department
Genetic variations of Narcissus tazetta and selected Narcissus cultivars based on the sequence analysis of nrITS and trnL‑IS‑trnF regions
Date of this Version
Genet Resour Crop Evol https://doi.org/10.1007/s10722-022-01521-4
Narcissus tazetta var. chinensis (N. taz. var. chi.) accessions collected from the coastal areas of China, Korea, and Japan were compared with accessions of lower ranks of Narcissus tazetta (N. taz.) from Italy to study the genetic variations and with several morphologically similar cultivars available in the trade. Sequences of the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (nrITS) dendrogram and the chloroplast trnL-IS-trnF intergeneric spacer-gene (cpIS) were analyzed by maximum likelihood (ML) and posterior probability using STRUCTURE program. All single fower form of N. taz. var. chi. collected from China, Korea, and Japan are very closely related based on the sequence analyses of cpIS and nrITS, regardless whether it is a single or double fower. Single fower N. taz. var. chi. from China, Korea, and Japan were not related to those from several lower ranks collected from Italy including N. taz. subsp. italicus. The double fower N. taz. var. chi. accessions were not related to any other morphologically similar double fower commercially available cultivars. The correlation between the ML dendrogram and the posterior probability analysis for cpIS are generally consistent with most N. taz. var. chi. accessions. However, significant differences between the clustering by ML dendrogram and posterior probability analysis for nrITS were observed, suggesting that posterior probability analysis is considered more valuable than ML dendrogram to study the genetic variations. The nomenclature of the lower ranks of N. taz. should be examined further.
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