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Thesis (M.S.)—University of Nebraska—Lincoln, 1957. Department of Dairy Husbandry.


Copyright 1957, the author. Used by permission.


Considerable work has been done by various investigators in the past ten years to develop a diluter for bovine semen that will maintain life and fertilizing capacity for longer periods of time than will the common egg yolk-buffer diluters. Heated homogenized whole milk and heated skimmilk have been shown to maintain motility, life, and fertilizing capacity of bovine semen equal to egg yolk-buffer extenders.

The purpose of this study was to try and develop a diluter superior to egg yolk-citrate for preserving livability and motility of bovine semen. The effects upon spermatozoan livability and motility of the additions of glycerol, glucose, glycine, and cysteine hydrochloride to egg yolk-citrate, heated homogenized whole milk, heated skimmilk, and reconstituted instant nonfat dry milk solids were investigated and reported in this paper.

Advisor: A. B. Schultze