Date of this Version
Thesis (M.S.)—University of Nebraska—Lincoln, 1957. Department of Dairy Husbandry.
Considerable work has been done by various investigators in the past ten years to develop a diluter for bovine semen that will maintain life and fertilizing capacity for longer periods of time than will the common egg yolk-buffer diluters. Heated homogenized whole milk and heated skimmilk have been shown to maintain motility, life, and fertilizing capacity of bovine semen equal to egg yolk-buffer extenders.
The purpose of this study was to try and develop a diluter superior to egg yolk-citrate for preserving livability and motility of bovine semen. The effects upon spermatozoan livability and motility of the additions of glycerol, glucose, glycine, and cysteine hydrochloride to egg yolk-citrate, heated homogenized whole milk, heated skimmilk, and reconstituted instant nonfat dry milk solids were investigated and reported in this paper.
Advisor: A. B. Schultze