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Thesis (M.S.)--University of Nebraska--Lincoln, 1964. Department of Agronomy.


Copyright 1964, the author. Used by permission.


Phenylalanine deaminase activity was examined in the isogenic sweet-clover (Melilotus alba Decr.) lines differing in the Cu/cu alleles. No definite differences in the activity of this enzyme were demonstrated in the two genotypes. The fact that this enzyme is active in the plants of the cucu genotype provides some support for the existence of a block in the ortho-hydroxylation of trans-cinnamic acid in this genotype.

Attempts to demonstrate the enzymatic conversion of trans-cinnamic acid to o-coumaryl glucoside have been unsuccessful. Since no positive results were obtained, it appears that the postulated glucosidation of trans-cinnamic acid probably does not occur under the conditions used.

The results obtained indicate that crude enzyme preparations of CuCuBB plants are not capable of converting o-tyrosine to o-coumaric acid.

Advisor: Francis A. Haskins.