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Thesis (M.S.)—University of Nebraska—Lincoln, 1966. Department of Botany.


Copyright 1966, the author. Used by permission.


The synthesis of virus protein inside the living cell is not completely understood. From studies in cellular metabolism during the last decade it is known that protein biosynthesis, in which amino acids are united in a specific order, occurs at the ribosomes in the cytoplasm. Many workers have theorized that the virus protein is also synthesized around the cytoplasmic ribosomes. If this is the case, the ribosomes in virus infected cells should be different from those in healthy cells. In fact, if the cytoplasmic ribosomes act as centers for the synthesis of virus protein, more ribosomes and possibly different ribosomes or polyribosomes should be found in infected cells.

These experiments have been designed to investigate the quality and quantity of ribosomes in healthy bean leaves and compare them to the ribosomes in infected leaves. Ribosomes were extracted from healthy and infected leaves of Bountiful and Great Northern bean varieties. The pathogen, southern bean mosaic virus, produces a systemic infection on Bountiful bean and a local infection on Great Northern bean.

Advisor: M. K. Brakke