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Thesis (M.S.)—University of Nebraska—Lincoln, 1968. Department of Veterinary Science.


Copyright 1968, the author. Used by permission.


The object of this study was to determine if bentonite particles could be sensitized and used in a test to determine the presence of hog cholera antibodies. Since serum neutralization tests require approximately two days to complete, are relatively expensive to perform and therefore are impractical as a routine laboratory test for detecting swine infected with low virulence hog cholera virus, it is hoped this study will provide an alternative test.

For this study, ten pigs were purchased from a farmer who had not practiced hog cholera vaccination in the previous three years.There had been no evidence of hog cholera on this farm since vaccination had been discontinued. Eight pigs were vaccinated with 5 ml. of a commercially prepared Crystal Violet Hog Cholera Vaccine.Two unvaccinated controls were placed in the isolation room with the vaccinated pigs.Five pigs died of hog cholera following vaccination.The three vaccinated survivors and the two controls were challenged by intramuscular inoculation with 2 ml. of virulent Ames Strain Hog Cholera virus 46 days post vaccination.

Advisor: Marvin J. Twiehaus