Date of this Version
Thesis (M.S.)—University of Nebraska—Lincoln, 1971. Department of Biochemistry and Nutrition.
The mode of aminopeptidase production was studied in Gramvariable Bacillus subtilis cultures. This microorganism exhibited at least three peptidolytic activities: (a) an extracellular endopeptidase produced during logarithmic growth, (b) an aminopeptidase activity present in the cultural fluid after logarithmic growth, and (c) an aminopeptidase activity detectable in extracts of washed cells. This study clearly demonstrated that the three enzymes were distinct and that the aminopeptidase in the cultural fluid during stationary growth was a true extracellular enzyme.
Advisor: Fred W. Wagner