Institut für Biologie der Martin-Luther-Universität Halle-Wittenberg


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Erforschung biologischer Ressourcen der Mongolei (2021) band 14: 379-384.


Copyright 2021, Martin-Luther-Universität Halle Wittenberg, Halle (Saale). Used by permission.


The medicinal application of specific plants over the long period in traditional medicine, suggests the presence of biologically active substances in those plant species. Saussurea amara (L.) DC. is a wild plant with a high feeding value because of its high protein content, vitamins, and minerals. S. amara is used in the traditional Mongolian medicine for the treatment of hepato-biliary disorders. The goal of this study was to evaluate the antioxidant and antimicrobial activities of S. amara. The antioxidant capacity of a methanol extract of plants was evaluated by analyzing its DPPH free-radical scavenging activity and reducing power and determination of total phenolic compounds. In the experiment of DPPH, the IC50 for the commercial standard BHA was 4.4±0.41 μg/ml. S. amara has a weak antioxidant activity by DPPH, FRAP and TPC respectively that, the IC50 value was 346 ± 6.03 μg/ml, 12.90 ± 0.42 μg equivalent to 1 μg BHT and 49.03 ± 0.81μg equivalent to 1 μg pyrocatechol. Antimicrobial assays of the test extract were performed on three clinical microorganisms, including Gram-positive (Staphylococcus aureus KCTC 3881), Gram-negative (Escherichia coli KCTC 1039) bacteria and one fungus (Candida albicans KCTC 7965). S. amara has two antibacterial (S. aureus, E. coli) activities. S. amara has two antibacterial (Staphylococcus aureus, E. coli) activities by the DCM fraction with an inhibition zone of S. aureus of 38 ± 0.2 mm, and that of E. coli was 14 ± 0.1 mm. The water fraction of S. amara did not show not any antibacterial effect. The results implicate, that S. amara has a relatively high antibacterial activity and that continuative studies on its natural compounds would be promising.