Papers in the Biological Sciences


Document Type


Date of this Version



Zhou, Zhang, Liu & Zhou in Nucelic Acids Research (2005) 33 (Special issue); doi:10.1093/nar/gki360


Copyright 2005, Oxford University Press. Used by permission.


We have developed the following web servers for protein structural modeling and analysis at http:// THUMBUP, UMDHMMTMHP and TUPS, predictors of trans-membrane helical protein topology based on a mean-burial-propensity scale of amino acid residues (THUMBUP), hidden Markov model (UMDHMMTMHP) and their combinations (TUPS); SPARKS 2.0 and SP3, two profile– profile alignment methods, that match input query sequence(s) to structural templates by integrating sequence profile with knowledge-based structural score (SPARKS 2.0) and structure-derived profile (SP3); DFIRE, a knowledge-based potential for scoring free energy of monomers (DMONOMER), loop conformations (DLOOP), mutant stability (DMUTANT) and binding affinity of protein–protein/ peptide/DNA complexes (DCOMPLEX & DDNA); TCD, a program for protein-folding rate and transition-state analysis of small globular proteins; and DOGMA, a web-server that allows comparative analysis of domain combinations between plant and other 55 organisms. These servers provide tools for prediction and/or analysis of proteins on the secondary structure, tertiary structure and interaction levels, respectively.