RAPID THERMOCYCLER SYSTEM FOR RAPID AMPLIFICATION OF NUCLEIC ACIDS AND RELATED METHODS

Authors

Joel R. TerMaat, University of Nebraska-LincolnFollow
Scoltt E. Whitney, Lincoln, NE
Hendrik J. Viljoen, University of Nebraska-LincolnFollow
Matthew R. Kreifels, Omaha, NE

Date of this Version

8-22-2017

Citation

United States Patent No. 9,737,891 B2. Aug. 22, 2017

Comments

US govt doc. Public domain.

Abstract

A thermo cycling device and method of operating a thermocycler instrument, the instrument including a sample holder, at least one thermal cycling element, and at least one first and second temperature sensors, for causing the sample holder containing the at least one sample to undergo polymerase chain reaction amplification by repeated cycling between at least a denaturation heating stage and an aunealing cooling stage. The first temperature corresponding with the temperature of the sample holder is monitored using the at least one first temperature sensor, and a second temperature corresponding with the temperature external of the sample holder is monitored using the at least one second temperature sensor. Based upon the first temperature and the second temperature, the power that is delivered to the at least one thermal cycling element of the instrument is dynamically controlled.