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Exploring the phase during wound healing when fibrinogen (FBG) and fibronectin (FN) interact forming a small thin layer for cells to migrate and proliferate at the injury site is necessary for the primary building blocks and initial stages of skin recovery. The aim of this thesis is to illustrate that administrating a reasonable amount of (FN) and FBG, in a semi-organizational technique, to the wound site will encourage cell population and ECM formation, as well as, improve the wound healing process. WE hypothesized that FBG and FN interactions will configure the construction of dermal cells and their final fate to remodel and flourish the wound.
One section of this thesis will compare the functional integrity of the different forms of FNs. Plasma derived fibronectin (Pd-FN), although different in structure and composition could function similarly to the cellular-derived fibronectin (c-FN).
The short peptide sequence Arg-Gly-Asp-Ser (RGDS), expressed on the structure of FN, helps the molecule to interact with a wide variety of crucial dermal cell receptors known as integrins, The α,β combinations of integrins expressed on the cell’s surface are triggered by external stimulus. The interactions are the initial steps in skin regeneration and recovery. They are responsible for cell migration, differentiation, and proliferation. The interactions are also responsible for ECM formation and anchoring cells to the ECM.