Mark A. Griep

Associate Professor
614A Hamilton Hall
University of Nebraska-Lincoln

Current Research: My lab is searching for antibiotics that inhibit bacterial primase and helicase. These DNA replication enzymes are special to the lagging strand during DNA synthesis. The factors that are driving our search are both the rising numbers of antibiotic-resistant bacterial strains and bioweapons such as Bacillus anthracis and Francisella tularensis. We are using numerous approaches including fluorescence resonance energy transfer (FRET), mass spectrometric peptide mapping, stopped-flow kinetics, computer-aided drug screening. and high-throughput screening of compound libraries.




Allosteric regulation of the primase (DnaG) activity by the clamp-loader (τ) in vitro, Kiran Chintakayala, Cristina Machón, Anna Haroniti, Marilyn A. Larson, Steven H. Hinrichs, Mark A. Griep, and Panos Soultanas



Staphylococcus aureus primase has higher initiation specificity, interacts with single-stranded DNA stronger, but is less stimulated by its helicase than Escherichia coli primase, Scott A. Koepsell, Marilynn A. Larson, Christopher Frey, Steven H. Hinrichs, and Mark A. Griep



Myricetin inhibits Escherichia coli DnaB helicase but not primase, Mark A. Griep, Sheldon Blood, Marilynn A. Larson, Scott A. Koepsell, and Steven H. Hinrichs



Kinetics of the DNA polymerase pyrococcus kodakaraensis, Mark A. Griep, Casey A. Kotera, R.M. Nelson, and Hendrik J. Viljoen


Staphylococcus aureus Helicase but Not Escherichia coli Helicase Stimulates S. aureus Primase Activity and Maintains Initiation Specificity, Scott A. Koepsell, Marilynn A. Larson, Mark A. Griep, and Steven H. Hinrichs


DNA Polymerase Chain Reaction: A Model of Error Frequencies and Extension Rates, Hendrik J. Viljoen, Mark A. Griep, Scott Whitney, and Michael Nelson



The cytolethal distending toxin B sub-unit of Helicobacter hepaticus is a Ca2+- and Mg2+-dependent neutral nuclease, Rohana P. Dassanayake, Mark A. Griep, and Gerald Duhamel


A macroscopic kinetic model for DNA polymerase elongation and high-fidelity nucleotide election, Steve Viljoen, Mark A. Griep, Michael Nelson, and Hendrik J. Viljoen



Primase structure and function, Mark A. Griep