Use of NMR Metabolomics to Analyze the Targets of D-cycloserine in Mycobacteria: Role of D-Alanine Racemase

Authors

Steven M. Halouska, University of Nebraska-LincolnFollow
Ofelia Chacon, University of Nebraska - Lincoln
Robert J. Fenton, University of Nebraska - LincolnFollow
Denise K. Zinniel, University of Nebraska - LincolnFollow
Raul G. Barletta, University of Nebraska - LincolnFollow
Robert Powers, University of Nebraska-LincolnFollow

Date of this Version

2007

Citation

J Proteome Res. 2007 December ; 6(12): 4608–4614. doi:10.1021/pr0704332.

Comments

© 2007 American Chemical Society

Abstract

D-cycloserine (DCS) is only used with multi-drug resistant strains of tuberculosis because of serious side-effects. DCS is known to inhibit cell wall biosynthesis, but the in vivo lethal target is still unknown. We have applied NMR-based metabolomics combined with principal component analysis to monitor the in vivo affect of DCS on M. smegmatis. Our analysis suggests DCS functions by inhibiting multiple protein targets.