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Genetic mapping of the quantitative trait loci associated with nutritional and quality traits in melon (Cucumis melo L.) and soybean (Glycine max L.)
Micronutrient malnutrition is a global problem and enrichment of plant edible tissues with nutrients (biofortification) is a cost effective and sustainable approach to alleviate micronutrient malnutrition. This dissertation included two projects that focused on understanding the genetics underlying nutritional and quality aspects in melon and soybean to eventually help in biofortification. In the first study we used three soybean recombinant inbred line mapping populations that had a common founder parent and also constructed a joint linkage map for these populations. A total of 49 QTLs that affected seed nutritional composition were detected using analysis of individual mapping populations and joint linkage mapping. We identified several candidate genes that might contribute to these traits, including transporters, genes involved in metabolism, and a transcription factor. Seed weight and seed mineral concentrations were not highly correlated, suggesting the possibility of improving seed mineral concentrations without significant changes in seed weight. An inverse relationship between Nitrogen:Sulfur (N:S) ratio and most other minerals suggests the possibility of using N:S ratio as an indirect measure of seed mineral concentration in soybean breeding programs. The C940-fe (fefe) germplasm contains an unidentified iron chlorotic mutation in an orange-fleshed melon. A dual purpose mapping population of melon was developed using a green-fleshed snake melon and C940-fe, with objectives of mapping the fefe gene and horticultural traits. A total of 24 QTLs were associated with various fruit traits. Of these,18 QTLs were previously reported,. Six new QTLs that affected fruit quality and consumer acceptability were detected. A small region on linkage group 8 was found to be a hotspot to increase yield in melon. Interestingly, fefe mutation was also mapped to the same region on linkage group 8 using the genetic mapping information from two F2 mapping populations of melon.
Kastoori Ramamurthy, Raghuprakash, "Genetic mapping of the quantitative trait loci associated with nutritional and quality traits in melon (Cucumis melo L.) and soybean (Glycine max L.)" (2013). ETD collection for University of Nebraska - Lincoln. AAI3603572.