Identification of Differential Gene Expression Among Low Phytic Acid and High Grain Protein Genotypes in Winter Wheat (Triticum aestivum L.)
P. Stephen Baenziger
Date of this Version
Winter wheat (Triticum aestivum L.) is a major staple for human nutrition. However, the antinutrient phytic acid limits the nutrient absorption and bioavailability of the essential nutrients of iron and zinc. Low phytic acid mutant (lpa1-1) reduces the phytic acid content in the aleurone layer of wheat. In wheat breeding programs, grain protein concentration (GPC) is one of the crucial traits, because it affects the nutritional value and bread-making properties of wheat. The grain protein content-B1 (Gpc-B1) gene regulates positively for increasing protein, iron, and zinc concentrations in the grain. Recombinant inbred genotypes (RILs) were developed that contain both lpa1-1 and Gpc-B1 to improve nutrient availability. RNAseq was performed on developing seeds at three stages to determine how lpa1-1 and Gpc-B1 affect global gene expression relative to their wild type (WT) counterparts during the critical period of grain filling. The goal of the study is to identify the gene that is responsible for the reduction of phytate in the lpa1-1.
Advisor: P. Stephen Baenziger
A THESIS Presented to the Faculty of The Graduate College at the University of Nebraska In Partial Fulfillment of Requirements For the Degree of Master of Science, Major: Agronomy, Under the Supervision of Professor P. Stephen Baenziger. Lincoln, Nebraska: December, 2019
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