Investigating Antibiotic Resistance in Staphylococcus aureus and Streptococcus mitis-oralis by Nuclear Magnetic Resonance Based Metabolomics
Date of this Version
Metabolomics is rapidly growing in popularity as a valuable resource for drug discovery due to its ability to identify perturbations in metabolic pathways between healthy controls and drug treatment groups. Metabolomics can differentiate between different phenotypes resulting from genetic changes or in response to variations in environmental conditions. Conversely, genomics, transcriptomics, and proteomics can only provide insight into the outcome that could happen. Notably, metabolomics is commonly used to observe the effects of environmental stress on bacteria and between resistant and non-resistant strains of bacteria. The development of a protocol to grow Staphylococcus aureus in a blood-like environment was essential in identify the metabolic, growth, and survivability differences cause due to cultivation medium. The blood-like environment consist of tryptic soy broth without dextrose (TSB-DEX) with 55% Serum. Daptomycin-susceptible (DapS) and daptomycin-nonsusceptible (DapNS) strains of S. aureus were then cultured in the blood-like environment to identify metabolome differences. NMR and mass spectrometry were used in combination to provide a comprehensive characterization of metabolic perturbations. In this regards, adaptive metabolic differences between DapS and DapNS may be leveraged as potential therapeutic targets. NMR metabolomics was also used to characterize metabolic response for adaptive resistance strains of Streptococcus mitis-oralis to daptomycin.
Advisor: Robert Powers
A Thesis Presented to the Faculty of The Graduate College at the University of Nebraska In Partial Fulfillment of Requirement For the Degree of Master of Science, Major: Chemistry, Under the Supervision of Professor Robert Powers. Lincoln, Nebraska: July, 2020
Copyright 2020 Allison Arnold Parrett