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Five novel homologous acetate derivatives of long-chain secondary alcohols and a related ketone were tested for their efficacy as contact mating stimulants for Cochliomyia hominivorax Coquerel (Diptera: Calliphoridae). Full copulatory behaviour at a high percentage was found in tests with racemic 6-acetoxy-19-methylnonacosane at 2.5 – 20 μg using fertile males from three strains. Males of two strains responded nearly as well to 7-acetoxy-15-methylnonacosane, but an older strain first colonized in 1992 did not respond to this compound. Few or no copulatory responses were obtained to the other secondary alcohol acetates and a related ketone. These two acetate derivatives are the first sex pheromones identified in a calliphorid fly. The threshold of response was also tested, but could not be pinpointed.