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The status of resistance to three insecticides (permethrin, stirofos, and methoxychlor), relative to a laboratory-susceptible colony, was evaluated in Þeld populations of house flies, Musca domestica L., collected from two beef cattle feedlots in southeastern Nebraska. Topical application and residual exposure to treated glass surfaces were suitable methods for determining the resistance status of house flies to permethrin, stirofos, or methoxychlor. However, in most cases, residual exposure was more sensitive in resistance detection (i.e., higher resistance ratios). The field populations tested were moderately resistant to permethrin (RR = 4.9-fold and RR = 7.3-fold, for topical application and residual exposure, respectively) and extremely resistant to stirofos and methoxychlor (not accurately quantifiable be cause of low mortality at the highest possible concentrations or doses). Probable explanations for the resistance status of these house fly populations and implications for global feedlot fly management are discussed.