Molecular Differentiation of Alfalfa Weevil Strains (Coleoptera: Curculionidae)

Authors

S. J. Erney, University of Nebraska-Lincoln
K. P. Pruess, University of Nebraska-LincolnFollow
S. D. Danielson, University of Nebraska-Lincoln
T. O. Powers, University of Nebraska-LincolnFollow

Date of this Version

1996

Citation

Annals of the Entomological Society of America 89:6 (1996), pp. 804–811.

doi: 10.1093/aesa/89.6.804

Comments

Copyright © 1996 Entomological Society of America; published by Oxford University Press. Used by permission.

Abstract

Mitochondrial DNA was amplified and sequenced from eastern, western, and Egyptian strains of alfalfa weevil, Hypera postica (Gyllenhal). Eastern and Egyptian weevils differed at only 2 nucleotide sites in 1,031 base pairs sequenced; western weevils differed by 5% sequence divergence. Three restriction sites were identified which separated eastern and western haplotypes. No intrastrain polymorphism was detected in 150 weevils from Nebraska. Collections from Lincoln in eastern Nebraska and Scottsbluff in western Nebraska were fixed for the eastern and western haplotypes, respectively. Eastern and western haplotypes were found together in the same fields in a broad overlap region in central Nebraska.