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We report here the first occurrence in Florida of polygynous (multiple queen) colonies of the red imported fire ant (RIFA), Solenopsis invicta Buren. Polygynous strains of RIFA have been reported previously in Mississippi (Glancey et al. 1973 and Glancey et al. 1975), Texas (Hung et al. 1974 and Mirenda and Vinson 1982), Georgia and Louisiana (Fletcher 1983).