Date of this Version
Environmental Studies Undergraduate Student Thesis, University of Nebraska–Lincoln, 2015
The increasing occurrence of pharmaceuticals at low concentration and other “emerging contaminants” in the surface and ground water, has led to a critical need for a wide variety of methods for the accurate measurement of chemicals at low concentrations in a cost effective manner. The objective of this study was to develop a sample preparation method that allows the quantification of two pharmaceuticals (carbamazepine and diphenhydramine) commonly reported in treated municipal wastewater in plant tissue (leafy lettuce) extracts using Gas Chromatography-Mass Spectrometry (GC-MS). Solid phase extraction (SPE) was used to extract analytes while additional sample purification was attempted through the use of reverse phase C18-bonded silica environmental cartridges and polymeric HLBTM cartridges. Sensitivity for the instrumental method was determined to be 36.6ng/g for diphenhydramine and 12.3ng/g for carbamazepine with a standard deviation of 4.0920 and 12.2284, respectively.