Honors Program
Date of this Version
2022
Document Type
Article
Citation
Ismail, Randa. "Malate Dehydrogenase and Citrate Synthase Multi-enzyme Complex Interaction Affects Metabolism and Growth of Living Yeast Cell." Undergraduate Honors Thesis. University of Nebraska - Lincoln. 2022.
Abstract
The tricarboxylic acid (TCA) cycle includes many enzyme-enzyme interactions. The effects of these interactions on metabolite flux are not well understood. Citrate synthase (CS) and malate dehydrogenase (MDH) form a multi-enzyme complex that channels oxaloacetate (OAA) as an intermediate. The aim of the study is to assess the effect of disruption of the MDH-CS multi-enzyme complex interaction on metabolite flux and growth of living yeast cells. Analysis was done using growth curves with three carbon sources, enzymatic activity, and GC-MS metabolite. Growth curves showed insignificant difference in all three carbon sources; enzymatic activity assay showed that the mutation at arginine residues of CS located at 104 does not affect the activities and abundances of the MDH, and CS. Aspartic acid was used as it can be converted to OAA and affect the multi-enzyme complex, and it showed lower relative intensity in raffinose mutant.
Comments
Copyright Randa Ismail 2022.