Date of this Version
Znamenacek, L. 2022. Adaptation of an Enzyme Kinetics Experiment in a Biochemistry Lab Course Designed for Non-Majors. Undergraduate Honors Thesis. University of Nebraska-Lincoln.
This Honors thesis project sought to overhaul one of the labs used in Bioc 401: Elements of Biochemistry, a biochemistry lab course intended for students outside the major. This adaptation involved switching to a new enzyme in an enzyme kinetics experiment, replacing alkaline phosphatase (ALP) with malate dehydrogenase (MDH). Various tests were conducted to determine what experimental conditions were suitable to be carried out by students during a typical class period. Based on these findings, new student and TA manuals were created, and the presentation used in the lab session was adjusted. Manuals were also reviewed to add additional details that nonmajors may not pick up on in the protocols compared to manuals meant for biochemistry majors. These materials could then be kept and adjusted by the instructors to be used in future sections of the course.