Date of this Version
Jones, C. Identification and Characterization of Potential Monomeric Transcription Factors in Mycobacterium tuberculosis. Undergraduate Honors Thesis. University of Nebraska - Lincoln. 2023.
Tuberculosis is a dangerous and adaptable disease caused by the pathogen Mycobacterium tuberculosis (Mtb) that affects a significant population worldwide. Recent studies have increasingly focused on the pathogen's complex regulatory system, controlled by hundreds of transcription factors (TFs). To better understand the regulatory mechanisms of Mtb, this thesis aims to investigate unique monomeric TFs and potential coregulators such as Rv1830. Rv1830 belongs to the MerR transcriptional family and has no known structure besides its essential function in Mtb. This research utilizes sequence analysis, protein purification, protein expression testing, and structural modeling to identify potential DNA binding residues of Rv1830. These residues allow Rv1830 to interact with the promoter regions of other TFs. Results from this thesis research will enhance our understanding of the regulatory mechanisms of Mtb and contribute to future drug therapy development.