Use of fecal volatile organic compound analysis to discriminate between nonvaccinated and BCG-Vaccinated cattle prior to and after Mycobacterium bovis challenge

Authors

Christine K. Ellis, USDA National Wildlife Research CenterFollow
Somchai Rice, Iowa State University
Devin Maurer, Iowa State University
Randal S. Stahl, USDA-APHIS-Wildlife ServicesFollow
W. Ray Waters, USDA National Wildlife Research Center
Mitchell V. Palmer, USDA National Wildlife Research CenterFollow
Pauline Nol, USDA APHIS Wildlife Services
Jack C. Rhyan, USDA APHIS Wildlife Services
Kurt C. VerCauteren, USDA-APHIS-Wildlife ServicesFollow
Jacek A. Koziel, Iowa State University

Date of this Version

2017

Citation

Ellis CK, Rice S, Maurer D, Stahl R, Waters WR, Palmer MV, et al. (2017) Use of fecal volatile organic compound analysis to discriminate between non-vaccinated and BCGÐVaccinated cattle prior to and after Mycobacterium bovis challenge. PLoS ONE 12(7): e0179914. https://doi. org/10.1371/journal.pone.0179914

Comments

Open Access.

Abstract

Bovine tuberculosis is a zoonotic disease of global public health concern. Development of diagnostic tools to improve test accuracy and efficiency in domestic livestock and enable surveillance of wildlife reservoirs would improve disease management and eradication efforts. Use of volatile organic compound analysis in breath and fecal samples is being developed and optimized as a means to detect disease in humans and animals. In this study we demonstrate that VOCs present in fecal samples can be used to discriminate between non-vaccinated and BCG-vaccinated cattle prior to and after Mycobacterium bovis challenge.