Divergent neural and endocrine responses in wild-caught and laboratory-bred Rattus norvegicus

Authors

Joanna Jacob, University of Richmond
Sally Watanabe, University of Richmond
Jonathan Richardson, University of Richmond
Nick Gonzales, University of Richmond
Emily Ploppert, University of Richmond
Garet Lahvis, Harvard Law School
Aaron Shiels, USDA APHIS National Wildlife Research Center
Sadie Wenger, University of Richmond
Kelly Saverino, University of Richmond
Janhavi Bhalerao, University of Richmond
Brendan Crockett, University of Richmond
Erin Burns, University of Richmond
Olivia Harding, University of Richmond
Krista Fischer-Stenger, University of Richmond
Kelly Lambert, University of RichmondFollow

Date of this Version

8-26-2022

Citation

Behavioural Brain Research 432 (2022) 113978

doi:10.1016/j.bbr.2022.113978

Comments

U.S. government work

Abstract

Although rodents have represented the most intensely studied animals in neurobiological investigations for more than a century, few studies have systematically compared neural and endocrine differences between wild rodents in their natural habitats and laboratory strains raised in traditional laboratory environments. In the current study, male and female Rattus norvegicus rats were trapped in an urban setting and compared to weight-and sex-matched conspecifics living in standard laboratory housing conditions. Brains were extracted for neural assessments and fecal boli were collected for endocrine [corticosterone and dehydroepiandrosterone (DHEA)] assays. Additionally, given their role in immune and stress functions, spleen and adrenal weights were recorded. A separate set of wild rats was trapped at a dairy farm and held in captivity for one month prior to assessments; in these animals, brains were processed but no hormone data were available. The results indicated that wild-trapped rats exhibited 31% heavier brains, including higher densities of cerebellar neurons and glial cells in the bed nucleus of the stria terminalis. The wild rats also had approximately 300% greater spleen and adrenal weights, and more than a six-fold increase in corticosterone levels than observed in laboratory rats. Further research on neurobiological variables in wild vs. lab animals will inform the extensive neurobiological knowledge base derived from laboratory investigations using selectively bred rodents in laboratory environments, knowledge that will enhance the translational value of preclinical laboratory rodent studies.