Liquid Chromatographic Determination of 4,4'-Dinitrocarbanilide, the Active Component of the Infertility Agent Nicarbazin, in Chicken, Duck, Goose, and Snake Eggs

Authors

Thomas M. Primus, USDA-APHIS-Wildlife Services
Dennis J. Kohler, USDA/APHIS/WS National Wildlife Research CenterFollow
Margaret A. Goodall, USDA-APHIS-Wildlife Services
Christi Yoder, USDA-APHIS-Wildlife Services
Thomas Mathies, USDA-APHIS-Wildlife Services
Lowell Miller, USDA-APHIS-Wildlife ServicesFollow
John J. Johnston, USDA-APHIS-Wildlife ServicesFollow
Kurt C. Vercauteren, USDA-APHIS-Wildlife ServicesFollow

Date of this Version

1-1-2003

Comments

Published by Journal of AOAC International; Vol. 86, No. 6, 2003.

Abstract

4,4'-Dinitrocarbanilide (DNC) was extracted from chicken, duck, goose, and snake eggs and isolated by reversed-phase liquid chromatography. DNC was detected by ultraviolet absorbance at 347 nm and quantitated by comparison with a calibration standard. Recoveries of DNC from fortified control chicken, duck, goose, and snake egg samples were determined for DNC levels of 0.16, 10, and 16 μg/g. The mean recoveries from chicken, duck, goose, and snake eggs were 92±4, 88±9, 87±7, and 95 ±6%, respectively. The method limits of detection for DNC in chicken, duck, goose, and snake eggs ranged from 0.015 to 0.035 μg/g. The reported method is much simpler than and equally efficient as previous methods developed for the determination of DNC residues in egg contents.